Effect of propofol on thrombospondin-1 expression in cultured newborn rat cortical astrocytes

VernacularTitle:丙泊酚对新生大鼠大脑皮层星形胶质细胞合成凝血酶敏感蛋白1的影响
Author: Yu ZHANG ¹ ; Yu LI ; Weiguang LI ; Chenggang ZHANG ; Tiehu YE
Author Information

1. 中国医学科学院//北京协和医院麻醉科
Keywords: propofol; astrocyte; thrombospondin-1
From: Journal of Southern Medical University 2013;(9):1316-1320
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of propofol on the expression of thrombospondin-1 (THBS-1) mRNA and protein in purified newborn rat cortical astrocytes in vitro. Methods Astrocytes were isolated from newborn rat cortex and grown in culture before exposure to propofol at 3, 10, 30, 100 or 300μmol/L for 6 h, 12, or 24 h. The mRNA level of THBS-1 was detected by RT-PCR, and the protein level of THBS-1 was detected by immunofluorescence cytochemistry and Western blotting. Results Propofol exposure caused significantly upregulated THBS-1 level in cultured astrocytess (P<0.05) to a level about 1.3 times higher than that in control cells. The mRNA and protein levels of THBS-1 in cultured rat cortical astrocytes were upregulated by exposures to 10, 30 and 100 μmol/L propofol (P<0.01). High expression of THBS-1 mRNA and protein was detected in the cells with exposures for different durations (P<0.05), especially in the 12 h group (P<0.01). Conclusion Propofol at clinically relevant concentrations can modulate the level of THBS-1 secreted by astrocytes of rat cerebral cortex in vitro.