Genetic characteristics of human adenovirus in severe acute respiratory infection cases in Luohe city, Henan province from 2017 to 2019
10.3760/cma.j.cn112866-20220402-00081
- VernacularTitle:2017—2019年河南省漯河市严重急性呼吸道感染病例人腺病毒基因特征分析
- Author:
Jianlin CAI
1
;
Jin XU
;
Sheng ZHAO
;
Zhibo ZHANG
;
Ying LIU
;
Yi FENG
;
Jie JIANG
;
Aili CUI
;
Yan ZHANG
;
Wenbo XU
;
Zhen ZHU
;
Naiying MAO
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 国家卫生健康委员会医学病毒和病毒病重点实验室，北京 102206
- Keywords:
Severe acute respiratory tract infections;
Human adenovirus;
Genotyping;
Genetic characteristics
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(4):385-396
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the genetic characteristics of human adenovirus (HAdV) in severe acute respiratory infection (SARI) cases in Luohe city, Henan province, China.Methods:After viral isolation of HAdV-positive specimens identified in SARI cases from October 2017 to February 2019, the Loop2 region of the Hexon gene was amplified and determined to initially identify the virus type. Then, based on the preliminary screening result, the full length of the sequences of the three target genes (Penton base, Hexon and Fiber) of the viral strains were amplified using specific primers for each HAdV type, and phylogenetic and sequence homology analyses were performed with the prototype strains and the representative strains of the corresponding types at home and abroad to identify the types of viral strains and understand their genetic characteristics.Results:A total of 18 viral isolates were obtained from 27 HAdV-positive throat swab specimens from 783 SARI cases in Luohe city, Henan province, and the molecular typing result showed that these strains belonged to species B (HAdV-3, HAdV-7 and HAdV-55), species C (HAdV-1, P1H2F2, Px1/Ps3H5F5, P89H5F5 and HAdV-6) and species E (HAdV-4). Among them, the highest positive detection rate was found for species C HAdV-1 isolates (33.3%), followed by species B HAdV-3 (22.2%) and species C P1H2F2 (11.1%). The four HAdV strains in this study (HAdV-3, HAdV-4, HAdV-7 and HAdV-55) were characterized by significantly conserved and stable in time and space; while three patterns of genetic recombination (P1H2F2, Px1/Ps3H5F5 and P89H5F5) were identified for HAdV-C strains in this study, and their potential public health significance needed to be confirmed by further studies.Conclusions:The HAdV infection of SARI cases in Luohe city, Henan province during 2017-2019 was dominated by species C, followed by species B and species E. These data provided a scientific basis for the prevention and control of local adenovirus-associated infectious diseases.
