Tick-borne encephalitis virus infection caused astrocyte activation and increased RANTES expression in brain tissue
10.3760/cma.j.cn112866-20220121-00014
- VernacularTitle:蜱传脑炎病毒感染引起脑组织星形胶质细胞活化和RANTES表达水平升高
- Author:
Hui PENG
1
;
Xuejuan LIU
;
Binbin ZHU
;
Zeng LI
;
Hanzhong WANG
;
Baojing LU
Author Information
1. 安徽医科大学基础医学院，合肥 230032
- Keywords:
Tick-borne encephalitis virus;
Neuroinflammation;
RANTES
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(4):371-377
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the possible pathogenic mechanism of the central nervous system by studying the neurodegenerative diseases caused by tick-borne encephalitis virus (TBEV) infection in mice.Methods:The mice were intracranially inoculated with TBEV, 8 days later the mice were sacrificed, the brain tissues of all mice were collected and observed after HE staining to verify if the model was successfully established; the expressions of neuronal nuclei (NeuN), glial fibrillary acidic protein (GFAP), proliferating cell nuclear antigen (PCNA) and reduced upon activation, normal T cell expressed and secreted (RANTES) in mouse brain tissue were detected by immunohistochemical method, the number of positive cells was counted and the gray value was analyzed to calculate the score.Immunofluorescence combined with laser scanning confocal microscopy were used to observe the distribution of NeuN and GFAP positive cells and the expression of RANTES and PCNA.Results:The number of NeuN-positive cells in the brain tissue of mice infected with TBEV decreased, while the GFAP-positive cells increased. Furthermore, astrocytes proliferated faster than neurons, and RANTES was mainly produced by astrocytes.Conclusions:TBEV infection could cause neurotoxic reactions in brain tissue, leading to nerve cells death and activating astrocytes which produces many RANTES.
