Effect of 3-bromopyruvate on mitochondrial membrane potential and apoptosis of human breast carcinoma SK-BR-3 cells
10.3969/j.issn.1673-4254.2013.09.10
- VernacularTitle:3-溴丙酮酸诱导人乳腺癌SK-BR-3细胞凋亡及对细胞线粒体膜电位的影响
- Author:
Yuanyuan ZHANG
1
;
Zhe LIU
;
Qianwen ZHANG
;
Zhenhua CHAO
;
Pei ZHANG
;
Fei XIA
;
Chenchen JIANG
;
Hao LIU
;
Zhiwen JIANG
Author Information
1. 蚌埠医学院药学系 安徽省生化药物工程技术研究中心
- Keywords:
breast cancer cells;
3-bromopyruvate;
glycolysis;
ATP;
reactive oxygen
- From:
Journal of Southern Medical University
2013;(9):1304-1307
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of glycolysis inhibitor 3-bromopyruvate (3-BrPA) in inducing apoptosis of human breast carcinoma cells SK-BR-3 and the possible mechanism. Methods MTT assay was used to detect the growth inhibition induced by 3-BrPA in breast cancer cells SK-BR-3. The apoptotic cells were detected by flow cytometry with propidium iodide (PI). ATP levels in the cells were detected by ATP assay kit, and DHE fluorescent probe technique was used to determine superoxide anion levels; the mitochondrial membrane potential was assessed using JC-1 staining assay. Results MTT assay showed that the proliferation of SK-BR-3 cells was inhibited by 3-BrPA in a time- and concentration-dependent manner. Exposure to 80, 160, and 320μmol·L-1 3-BrPA for 24 h resulted in cell apoptosis rates of 6.7%, 22.3%, and 79.6%, respectively, and the intracellular ATP levels of SK-BR-3 cells treated with 80, 160, 320μmol·L-1 3-BrPA for 5 h were 87.7%, 60.6%, and 23.7%of the control levels. 3-BrPA at 160μmol·L-1 increased reactive oxygen levels and lowered mitochondrial membrane potential of SK-BR-3 cells. Conclusion 3-BrPA can inhibit cell proliferation, reduce the mitochondrial membrane potential and induce apoptosis in SK-BR-3 cells, the mechanism of which may involve a reduced ATP level by inhibiting glycolysis and increasing the reactive oxygen level in the cells.
