Preparation and identification of polyclonal antibody against methyl-accepting chemotaxis signal transduction protein of Helicobacter hepaticus

VernacularTitle:肝螺杆菌甲基基团趋化信号转导蛋白多克隆抗体的制备及鉴定
Author: Shengping HE ¹ ; Yahua CHEN ; Yingying ZHAO ; Jide WANG ; Yang BAI
Author Information

1. 南方医科大学南方医院胸心血管外科
Keywords: Helicobacter hepaticus; signal transduction protein; antibody preparation
From: Journal of Southern Medical University 2013;(9):1295-1298
CountryChina
Language:Chinese
Abstract: Objective To prepare the polyclonal antibody against methyl-accepting chemotaxis signal transduction protein (MCP) of Helicobacter hepaticus (H.hepaticus). Methods The recombinant plasmid pET22b+/MCP was transformed into E.coli BL2l(DE3) to express the fusion protein His-rhMCP under the induction of IPTG. The fusion protein was purified and the antibody was obtained by immunizing rabbits. The titer of the polyclonal antibody was tested by indirect ELISA, and the specificity of the antibody was identified based on Western blotting using the prepared cell surface proteins (CSPs) of the bacteria. Results The fusion protein was successfully expressed, and the titer of the antibody reached 1∶32 000. Western blotting indicated that the antibody could specifically bind to CSPs and His-rhMCP. Conclusion The antibody with a high titer and specificity was prepared to facilitate further study of the pathogenicity and epidemiology of H.hepaticus in human.