Viral nucleic acid tests and application
10.3760/cma.j.cn112866-20220316-00060
- VernacularTitle:病毒核酸检测技术及临床应用
- Author:
Ran WANG
1
;
Linlin ZHANG
;
Jiao TIAN
;
Mengjia LIU
;
Zhengde XIE
Author Information
1. 国家儿童医学中心 首都医科大学附属北京儿童医院 北京市儿科研究所感染与病毒研究室 中国医学科学院儿童危重感染诊治创新单元 国家呼吸系统疾病临床医学研究中心 儿科重大疾病研究教育部重点实验室 儿童呼吸道感染性疾病研究北京重点实验室，北京 100045
- Keywords:
Virus;
Nucleic acid test;
Clinical;
Application
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(3):337-345
- CountryChina
- Language:Chinese
-
Abstract:
Viral infectious diseases are a class of major diseases that seriously affect human life and health, and the causes of emerging infectious diseases are mostly viral pathogens. Rapid and accurate viral pathogen tests are of great significance for the clinical diagnosis and control of such infectious diseases. As an indispensable auxiliary diagnostic method, viral nucleic acid tests have the advantages of high sensitivity and specificity, etc., and have become an important method to identify the cause, determine the treatment schedule, and evaluate the treatment effect and prognosis. A series of method based on polymerase chain reaction, isothermal amplification technology or genome sequencing, respectively, have been successfully applied to viral nucleic acid detection. In this review, we will discuss the tests and applications of viral nucleic acid detection commonly used in clinical practice, as well as the issues that need attention in the interpretation of the result, to help the accurate diagnosis and treatment of viral infectious diseases.
