Performance of commercialized kits for detecting IgG antibody to measles virus
10.3760/cma.j.cn112866-20210812-00143
- VernacularTitle:商品化麻疹病毒IgG抗体检测试剂盒性能评价
- Author:
Daxing FENG
1
;
Wenhui WANG
;
Guangwei LI
;
Zhanpei XIAO
;
Yating MA
;
Wanyu LYU
;
Yanyang ZHANG
Author Information
1. 河南省疾病预防控制中心 河南省免疫预防医学重点实验室，郑州 450016
- Keywords:
Measles, IgG antibody;
Kit;
Performance
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(3):323-326
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the performance of four ELISA kits for detecting measles virus IgG antibody and to provide support for sero-epidemiologic survey.Methods:A total of 176 specimens were tested by four ELISA kits, respectively. The result of 4 kits were analyzed by the McNemar’s test. The values such as Kappa value, CV value, sensitivity, specificity, positive predictive value and negative predictive values were calculated and compared.Results:There was no significant difference among the result from kits and references, the χ2 were 0.333, 2.000, 1.000 and 0 respectively. The Kappa value of kit C was the highest (0.976), the sensitivity of kit A, C and D were the highest (99.34%), the specificity of kit B and C were the highest (100%), the positive predictive value of kit B and C were the highest (100%) and the negative predictive value of kit C were the highest (96.00%), there was no significant difference among the indicators from 4 kits ( P >0.05). The CV value of kit D was the lowest (5.30%). The correlation coefficient between the result from kit C and D was 0.639 ( P<0.01). Conclusions:The result showed that the 4 kits were suitable for serum epidemiologic survey.
