Evaluation of high-throughput methods for the detection of neutralizing antibodies in serum samples from individuals infected with 2019-nCoV based on a microneutralization test
10.3760/cma.j.cn112866-20211109-00198
- VernacularTitle:基于微量中和试验的新冠感染者血清样本中和抗体高通量检测方法评价
- Author:
Xiaoxiao KONG
1
;
Shihan ZHANG
;
Zhifeng LI
;
Huiyan YU
;
Xin ZOU
;
Hua TIAN
;
Ke XU
;
Qigang DAI
;
Yin CHEN
;
Jingxian LIU
;
Hongxiong GUO
;
Xiling GUO
;
Changjun BAO
;
Liguo ZHU
Author Information
1. 江苏省疾病预防控制中心 国家卫生健康委员会肠道病原微生物重点实验室，南京 210009
- Keywords:
Microneutralization test;
Individuals infected with 2019-nCoV;
Neutralizing antibody;
High-throughput
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(3):306-310
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the detection ability of two kinds of high-throughput method to determine neutralizing antibodies based on a microneutralization test (MNT).Methods:Serum samples were collected from the early phase and follow-up period (117 samples in total) for neutralizing antibody testing. They were tested using MNT, pseudovirus neutralization assay (PBNA), competitive inhibition assay (including enzyme-linked immunosorbent assay (ELISA) and chemiluminescence immunoassay (CLIA)) to evaluate the correlation coefficients and threshold values for the effectiveness of these high-throughput neutralizing antibody assays.Results:The correlation coefficients for PBNA, ELISA, and CLIA relative to MNT were 0.760, 0.778, and 0.725, respectively, for individuals infected with 2019-nCoV. The area under the ROC curve was 0.901 for a cutoff value of 50 for the PBNA assay, 0.934 for a cutoff value of 1∶8 for the ELISA assay and 0.838 for a cutoff value of 1.28AU/ml for the CLIA assay when the threshold value for the microneutralization test was taken as 1: 10 (less than 1: 10 is considered negative).Conclusions:The high-throughput method for the detection of COVID-19 neutralizing antibodies are scientific and feasible, and provide an important technical tool for the regular prevention and control of the epidemic.
