Characteristics of the whole genome of Banna virus carried by Culicoides in Cangyuan county, Yunnan province
10.3760/cma.j.cn112866-20211224-00214
- VernacularTitle:云南省沧源县蠓虫携带版纳病毒全基因核苷酸序列特征分析
- Author:
Lifen YANG
1
;
Juan WANG
;
Weihong YANG
;
Guopeng KUANG
;
Yun FENG
Author Information
1. 大理大学公共卫生学院，大理 671000
- Keywords:
Banna virus;
Culicoides;
Phylogenetic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(3):289-295
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the whole genome and genetic evolution characteristics of the Banna virus carried by Culicoides in Cangyuan County. Methods:Culicoides were collected in Cangyuan county in 2018. After grinding, the supernatant was inoculated onto BHK-21 cells for virus isolation. At the same time, the virus carrying status of Culicoides was detected by metatranscriptomics technology. Perl, Mafft and other bioinformatics softwares were used for sequence alignment, homology and molecular genetic evolution analysis. Results:Through metatranscriptomics method, the whole genome of Banna virus CYC1801 strain was obtained. Analysis of the whole genome showed that segment 4 and segment 7 are closely related to the Vietnamese strains, other segments are closely related to China strains. According to the phylogenetic analysis of the segment 12 gene, CYC1801 and the A2 gene subtype are in the same evolutionary branch and belong to the A2 gene subtype of Banna virus.Conclusions:Banna virus was carried by Culicoides collected in Cangyuan county, which may cause disease epidemic as a vector, so monitoring and prevention should be strengthened.
