Analysis on characteristics of virus isolation and unique recombinant forms from untreated HIV-1 infected patients in three provinces of China
10.3760/cma.j.cn112866-20220325-00069
- VernacularTitle:中国三省份未治疗HIV-1感染者临床毒株分离及其独特重组型的特征
- Author:
Lijie WANG
1
;
Yi FENG
;
Lin HE
;
Ruolei XIN
;
Yan WANG
;
Shu LIANG
;
Yibo DING
;
Shujia LIANG
;
Yuanyuan ZHANG
;
Caiqin HU
;
Yuhua RUAN
;
Hui XING
;
Kunxue HONG
;
Yiming SHAO
;
Liying MA
Author Information
1. 中国疾病预防控制中心艾滋病性病预防控制中心 传染病预防控制国家重点实验室，北京 102206
- Keywords:
HIV-1;
Route of transmission;
Men who have sex with men;
Virus isolation;
Unique recombinant forms
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(3):263-269
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the characteristics of viral isolation and unique recombinant from untreated HIV-1 patients infected through sexual transmission and injection drug use, so as to provide evidence for understanding the biological characteristics and precise prevention and control of HIV-1 infection in different transmission routes.Methods:In view of the different HIV-1 transmission risks, newly diagnosed untreated HIV-1 patients from Beijing, Guangxi and Sichuan were carefully selected. Venous blood was collected to detect the viral load and CD4 + T cell count, and peripheral blood lymphocytes were isolated for virus isolation. Viral RNAs were extracted from the virus supernatant, and the near full-length genome sequences were obtained using in-house method, then the recombination patterns were determined. Results:Among the 65 HIV-1 infection, 32(49.2%), 20(30.8%) and 13(20.0%) were infected via men who have sex with men (MSM), heterosexual and injection drug use (IDU), respectively; genotypes mainly included 26(40.0%) CRF07_ BC, 23(35.4%) CRF01_ AE, and 9(13.8%) unique recombinant types (URFs). A total of 46 HIV-1 clinical strains were isolated. The positive rate of HIV-1 isolation was significantly negatively correlated with CD4 + T cells ( X2=4.22, P=0.04), but positively correlated with viral load ( X2=22.4, P<0.001); the multi-variate generalized estimating equations(GEE) model analysis of HIV-1 P24 antigen content showed similar result. In addition, GEE model showed a positive correlation between viral P24 antigen content and virus-producing culture time (52.14, 95% CI: 9.42~94.87, P=0.017). Viral growth curve analysis showed that the level of viral P24 antigen in MSM Group was significantly higher than that in heterosexual group and IDU group (adjusted P values were p<0.01 and P<0.05, respectively), on the 14th day after culture. The proportion of URFs in MSM Group was higher than that in heterosexual group, and the recombinant breakpoints in MSM Group were more than that in heterosexual group. Conclusions:MSM population was more sensitive to HIV-1 virus isolation; there was unique diversity of recombinant forms of HIV-1 among those with sexually transmitted infections, especially in the MSM population.
