Viral nucleic acid detection technology based on graphene field effect transistor
10.3760/cma.j.cn112866-20220418-00093
- VernacularTitle:基于石墨烯场效应晶体管的病毒核酸检测技术
- Author:
Yungen WU
1
;
Yunqi LIU
;
Dacheng WEI
Author Information
1. 复旦大学材料科学系 分子材料与器件实验室，上海 200433
- Keywords:
Virus;
Nucleic acid;
Graphene field effect transistor;
Sensitivity
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(3):252-257
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This work developed a novel and convenient detection method which realized rapid and sensitive detection of the viral nucleic acid.Methods:Here we established a novel nucleic acid detection method based on a graphene field effect transistor (g-FET). By anchoring a chemical molecule on the sensing interface and then modifying with the highly sensitive DNA tetrahedral probes, it realized accomplish highly sensitive detection of the viral nucleic acid. By measuring the transfer curve of the devices, it can make the biological signal of the hybridization for the probe molecule and the target RNA converted into an electrical signal of the g-FET devices. Then through the signal amplification of the field effect transistor (FET) device, it realized a high-sensitive detection of the viral RNA.Results:The DNA tetrahedron probe we designed was targeted at the ORF1 ab gene region of the 2019 novel coronavirus (2019-nCoV) RNA. The target RNA was hybridized and bound by the principle of base-pair complementation. Then we tested the 2019-nCoV simulative RNA samples with different concentrations in saliva, when the concentration of target RNA increased, the Dirac point of the devices presented a regular leftward offset. The limited of detection concentration of this sensor can reach 0.05 copy/μl, and the response time was shorter than 5 minutes in 100 μl volume of tested liquid. Conclusions:In this work, we developed a novel g-FET sensor based on DNA tetrahedral probes, which realized a rapid and sensitive detection of viral nucleic acid.
