Development and evaluation of the detection method of rapid RT-PCR assays for severe fever with thrombocytopenia syndrome virus, dengue virus and hantaan virus
10.3760/cma.j.cn112866-20210618-00099
- VernacularTitle:SFTS病毒、登革病毒、汉滩病毒快速荧光定量RT-PCR检测方法的建立及评价
- Author:
Tingting HU
1
;
Yu ZHU
;
Aqian LI
;
Lina SUN
;
Xiaoxia HUANG
;
Wei WU
;
Chuan LI
;
Qin WANG
;
Jiandong LI
;
Dexin LI
;
Shiwen WANG
;
Yan LIU
;
Mifang LIANG
;
Xiaofang WANG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 国家卫生健康委生物安全重点实验室，北京 102206
- Keywords:
Severe fever with thrombocytopenia syndrome virus;
Dengue virus;
Hantaan virus;
Rapid fluorescence quantitative real-time PCR
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(2):230-235
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a quick on-site emergency detection method for severe fever with thrombocytopenia syndrome virus (SFTSV), dengue virus (DENV), and hantaan virus (HTNV).Methods:This research was based on the traditional TaqMan fluorescent probe technology, using the domestic rapid one-step quantitative RT-PCR kit, combined with the Magnetic induction cycler (Mic) qPCR instrument. The detection limit, specificity and repeatability of this method were evaluated by simulated samples, other virus infected samples and normal human blood samples.Results:Compared with the traditional RT-PCR assay, the required time of this method was greatly shortened, and the detection can be completed within 35 minutes. The limit of quantitation for SFTSV, DENV and HTNV are less than 100copies/PCR. No nonspecific amplification was found in the simulated negative samples and other virus infected samples. All the simulated positive sample for verification could be detected, and coefficient of variation Ct value of each group was less than 4%. Conclusions:The rapid fluorescence quantitative RT-PCR assays have certain application prospects for on-site emergency detection, and provide important technical supports and new directions for the prevention and control of common hemorrhagic fever viruses.
