Isolation and identification of Culex pipiens pallens densovirus from Culex tritaeniorhynchus in Shaanxi province in 2019
10.3760/cma.j.cn112866-20220226-00046
- VernacularTitle:2019年陕西省三带喙库蚊中淡色库蚊浓核病毒的分离与鉴定
- Author:
Xueping XU
1
;
Shihong FU
;
Bin LI
;
Sichao YING
;
Kai NIE
;
Xingzhou LI
;
Huanyu WANG
;
Guodong LIANG
Author Information
1. 佳木斯大学公共卫生学院，佳木斯 154007
- Keywords:
Culex pipiens pallens Densovirus;
Culex tritaeniorhynchus;
Phylogenetic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(2):225-229
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To perform virological and molecular biological identification of the virus (SX1943) isolated from specimens of Culex tritaeniorhynchus in Shaanxi province in 2019. Methods:Mosquito ground fluid was inoculated into tissue culture cells, and isolates were made virological and molecular genetic analysis.Results:A virus isolate (SX1943) was obtained from a specimen of Culex tritaeniorhynchus collected in Shaanxi province in 2019, which developed significant cytopathic effect (CPE) on day 3 after inoculation into C6/36 cells, manifested as cell pyknosis, aggregation and shedding, and the isolate could be stably passaged. However, no significant CPE was observed after three consecutive passages in BHK-21 cells. Electron microscopy (negative staining) of C6/36 cells inoculated with SX1943 virus showed a large number of round virus particles, about 25 nm in diameter. The result of amplification and sequencing of the SX1943 viral genome showed that the total length of the viral genome sequence was 3 594 nt, encoding a total of three proteins, which were non-structural proteins (NS1 and NS2) and capsid proteins (VP), and the gene lengths of the three proteins were 2 376 nt, 1 098 nt and 1 136 nt, respectively. Phylogenetic analysis of the virus revealed that the SX1943 virus were in the same evolutionary clade as Culex pipiens pallens Densovirus (CppDNV) in the genus Brevidensovirus in the subfamily Densovirus, and the above result suggested that SX1943 virus was CppDNV. Conclusions:CppDNV was isolated from Culex tritaeniorhynchus in Shaanxi province.
