Isolation and identification of human rhinovirus strains in children patients with pneumonia in Qingdao in 2020
10.3760/cma.j.cn112866-20210928-00174
- VernacularTitle:2020年冬季青岛肺炎患儿人鼻病毒毒株的分离与鉴定
- Author:
Yiqiu WAN
1
;
Fachun JIANG
;
Juan SONG
;
Jing JIA
;
Xia WANG
;
Yanhai WANG
;
Guoyong MEI
;
Ru CAI
;
Jun HAN
Author Information
1. 安徽理工大学医学院，淮南 232001
- Keywords:
Human rhinovirus;
Virus isolation;
Phylogenetic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(2):219-224
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To Isolate and identify human rhinovirus (HRV) in hospitalized children with pneumonia in Qingdao in 2020.Methods:A total of 98 throat swab samples were collected from hospitalized children with pneumonia in 2020. Common respiratory viruses were screened through RT-qPCR. The HRV positive samples were inoculated into H1-HeLa cells. Viruses with typical cytopathic effect (CPE) were identified by HRV specific RT-PCR. Subsequently, sequences of HRV-VP4/VP2 gene were used to construct phylogenetic trees and analyze homology with sequences of reference strains through MEGA software.Results:Among 98 hospitalized children with pneumonia, 11 samples were HRV positive in 98 throat swab samples. After the typical CPE appeared in HeLa cells, two strains of HRV were identified by specific RT-PCR. The HRV-A28 and HRV-A58 were confirmed by comparison and analysis of VP4/VP2 sequence. Phylogenetic tree found that the isolated HRV-A28 strain was genetically close to strains of Singapore in 2011, of Tunisia in 2017, and Kenya in 2017. The isolated HRV-A58 strain was genetically close to the strains of Australia in 2009, Venezuela in 2011, Mongolia in 2011, and the United States in 2014.Conclusions:The HRV-A28 and HRV-A58 strains were isolated from the throat swabs of children patients with pneumonia in Qingdao.
