Establishment of 2019 novel coronavirus neutralizing antibody detection method based on quantum dotimmunochromatography
10.3760/cma.j.cn112866-20220223-00039
- VernacularTitle:基于量子点免疫层析技术的新型冠状病毒中和抗体检测方法建立
- Author:
Qiangling YIN
1
;
Hengqiang WANG
;
Tingyou CHEN
;
Yuanyuan QU
;
Lina SUN
;
Wei WU
;
Tao HUANG
;
Xiao XU
;
Tingting TIAN
;
Chuan LI
;
Dexin LI
;
Mifang LIANG
;
Shiwen WANG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所，北京 102206
- Keywords:
2019 novel coronavirus;
Quantum dot immunochromatography;
Neutralizing antibody
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(2):205-213
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a simple, rapid and low-cost 2019 novel coronavirus (2019-nCoV) neutralizing antibody detection method.Methods:The 2019-nCoV RBD specific immunoglobulin G (RBD-IgG) detection method was established based on the principle of quantum dot immunochromatography(QDs), and the detection was evaluated by using of sera from coronavirus disease 2019 (COVID-19) convalescent patients ( N = 97), vaccinated donors ( N = 82) and healthy donors ( N = 299). The suitability of fingertip blood was evaluated by matching blood samples with peripheral blood ( N=54). Results:The 2019-nCoV RBD-IgG detection method based on QDs was successfully established. The detection result of QDSs had strong correlation ( Spearman r > 0.73, P < 0.000 1) and good consistency ( Kappa=0.93, P < 0.01) with the result of micro-neutralization test(MNT). The sensitivity and specificity were 92% and 99%, respectively. There was high correlation ( Spearman r =0.932 6, P < 0.000 1) and no significant difference ( P=0.102 6) between result of fingertip blood and peripheral blood. Fingertip blood can be used as a surrogate sample for testing. Conclusions:The 2019-nCoV neutralizing antibody detection method established in this study can provide an immediate, efficient and low-cost method selection for the assessment of herd immunity status, and provide technical support for the herd immunity monitoring of 2019-nCoV vaccinated population and the prevention and control of the epidemic.
