Analysis of the etiological characteristics of hand, foot and mouth disease in Songjiang district of Shanghai in 2019
10.3760/112866-20220129-00021
- VernacularTitle:2019年上海市松江区手足口病A组肠道病毒病原特征分析
- Author:
Ling LIU
1
;
Jiajin WU
;
Xuefei QIAO
;
Jiayu WANG
;
Zhongxing SUN
;
Ning HAN
;
Fengsong SHENG
Author Information
1. 上海市松江区疾病预防控制中心微生物检验科，上海 201620
- Keywords:
Hand, foot and mouth disease;
Enterovirus;
Genotype;
Phylogenetic analysis;
Homology
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(2):189-194
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the characteristics of enterovirus (EV) VP1 gene from cases with hand, foot and mouth disease (HFMD) in Songjiang district of Shanghai in 2019.Methods:Samples from suspected HFMD cases were detected using real-time fluorescence reverse transcription polymerase chain reaction (RT-PCR). Human rhabdomyosarcoma (RD) cells were used for EV culture. VP1 genes of the isolated EV A species were sequenced. The sequences of nucleotide and amino acid of EV A species were used in phylogenetic and homology analysis by MEGA X software.Results:Totally 207 HFMD specimens were detected in 2019, of which 188 specimens were screened positive for EV. The positive rates of Coxsackievirus (CV)-A6, CV-A16, CV-A10 and CV-A4 were 47.34% (89/188), 41.49% (78/188), 3.72% (7/188) and 2.66% (5/188), respectively. Other EV species were 4.79% (9/188) positive and EV-A71 was not detected. During the summer epidemic peak of HFMD (May to July), B1 gene subtype of CV-A16 was detected more frequently, of which B1a and B1b evolutionary branches were prevalent together. The CV-A6 virus of D3a branch dominated in secondary peak of autumn and winter (September to December). CV-A10 and CV-A4 were sporadic and both respective strains belonged to subtypes of C2 gene. Compared with the prototype strains, the nucleotide (amino acid) sequence homologies of CV-A6, CV-A16, CV-A10 and CV-A4 VP1 genes were 79.22%-81.78% (95.97%~-97.19%), 62.70%-65.54% (90.10%-91.30%), 81.76%-82.65% (91.63% -92.03%) and 81.09%-81.79% (97.27%-97.67%), respectively.Conclusions:The diversity and complexity of HFMD pathogen epidemic increase the difficulty of HFMD prevention and control. The expansion of EV surveillance programs and the studies on the molecular epidemiology of EV are helpful for the prevention and control of HFMD.
