Molecular evolution of Culex flavivirus in Gansu province in 2011 and 2019
10.3760/cma.j.cn112866-20220226-00045
- VernacularTitle:2011和2019年甘肃省库蚊黄病毒分子遗传进化特征
- Author:
Bin LI
1
;
Bing ZHA
;
Shihong FU
;
Xueping XU
;
Sichao YING
;
Kai NIE
;
Xingzhou LI
;
Huanyu WANG
;
Guodong LIANG
Author Information
1. 佳木斯大学公共卫生学院，佳木斯 154007
- Keywords:
Culex;
Flavivirus;
Phylogenetic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(2):183-188
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To disclose the molecular genetic differences of Culex flavivirus among mosquitoes in Gansu province in 2011 and 2019.Methods:Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to obtain the nucleotide sequences of Culex flavivirus genomes from mosquitoes in Gansu province in 2011 and 2019, and viral molecular biology and bioinformatics method were used to analyze the genetic differences of the viruses.Results:Nucleotide sequences of 10 strains of Culex flavivirus were obtained, including 8 strains (all from Culex pipiens pallens) obtained in 2011 and 2 strains ( from Culex tritaeniorhynchus and Anopheles sinensis) in 2019. Homology analysis of nucleotide and amino acid sequences of virus E gene showed that the nucleotide sequence similarity and amino acid similarity of viruses isolated from Gansu in 2019 and 2011 ranged from 98.3%-100% and 95.4%-97.3%, respectively. Phylogenetic analysis of Culex flavivirus E gene sequence showed that two strains of Culex flavivirus isolated in Gansu province in 2019 (GS1975 and GS1976) and eight strains of Culex flavivirus isolated in 2011 all belonged to group B of genotype 1 of Culex flavivirus. Further analysis found that GS1975 virus isolated in 2019 was in a common evolutionary cluster with viruses isolated from Liaoning (2010 and 2011) and Inner Mongolia (2018), while GS1976 virus isolated in 2019 formed a coevolutionary cluster with viruses isolated from Inner Mongolia (2018) and Gansu (2011). Conclusions:Although both Culex flaviviruses isolated in Gansu province in 2011 and 2019 are genotype 1 virus, the two viruses isolated in 2019 distributed in two different evolutionary clusters, suggesting that the local mosquito virus genome changes over time, therefore, long-term monitoring of molecular differences is needed to carry out.
