Characteristics of HA, NA genes and antigenicity of two strains of Eurasian avian H1N1 swine influenza virus from two human cases in Gansu province
10.3760/cma.j.cn112866-20210508-00077
- VernacularTitle:甘肃省两例人感染欧亚类禽H1N1猪流感病毒的HA、NA基因特性和抗原性分析
- Author:
Hongyu LI
1
;
Ping WANG
;
Baodi LI
;
Zi LI
;
Xiyan LI
;
Lei YANG
;
Jia LIU
;
Minju TAN
;
Zixuan LIANG
;
Congshan XU
;
Minghui MA
;
Hua WANG
;
Miao WANG
;
Deshan YU
;
Dayan WANG
Author Information
1. 甘肃省疾病预防控制中心 甘肃省传染病病原学重点实验室，兰州 730000
- Keywords:
Eurasian avian H1N1 swine influenza;
Antigenicity;
Neuraminidase;
Gene characteristics
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(2):166-171
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the antigenicity and genetic characteristics of two strains of Eurasian avian H1N1 swine influenza virus (EAS-H1N1) isolated from 2 human cases found in Gansu province to provide scientific reference for disease prevention and control.Methods:Two cases of human infection with EAS-H1N1 discovered in Gansu province since February 2021 were analyzed for antigenicity and genome comparison, and their genome characteristics were analyzed using software such as Mega7.0.Results:Two human infections with EAS-H1N1 had related environmental exposure history. Two strains were obtained: A/Gansu-Xifeng/1143/2021 and A/Gansu-Xifeng/1194/2021. The two strains of HA, NA genes were all from the Eurasian avian H1N1 swine influenza virus, and are closely related to the human infection EAS-H1N1 virus in Hebei and Tianjin and the EAS-H1N1 virus isolated from the mink in Shandong; the HA receptor binding sites of 2 strains E190D and D225E mutations occurred in all spots, and there were no H274Y and N294S mutations in NA protein.Conclusions:Some important molecular mutations occurred in the genome of people infected with EAS-H1N1 virus in Gansu province, which may lead to increased virulence and potential human-to-human transmission. Strengthening the research and analysis of EAS-H1N1 gene characteristics will help to monitor the mutation and mutation of the virus at the molecular level and scientifically prevent and control the epidemic.
