Consistency analysis of serum viral specific antibody against respiratory viruses and nucleic acid determination tests in children with community acquired pneumonia
10.3760/cma.j.cn112866-20100917-00166
- VernacularTitle:儿童社区获得性肺炎血清免疫学病毒特异性抗体检测与呼吸道病毒核酸检测结果一致性分析
- Author:
Qianyu FENG
1
;
Lijuan YANG
;
Hongwei ZHAO
;
Yun ZHU
;
Zhengde XIE
;
Lili XU
Author Information
1. 国家儿童医学中心 首都医科大学附属北京儿童医院 北京市儿科研究所感染与病毒研究室 中国医学科学院儿童危重感染诊治创新单元 国家呼吸系统疾病临床医学研究中心 教育部儿科重大疾病研究重点实验室 儿童呼吸道感染性疾病研究北京市重点实验室，北京 100045
- Keywords:
Community acquired pneumonia in children;
Respiratory viruses;
Nucleic acid detection;
Serum specific antibody detection
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(2):150-154
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the consistency between the results of serum specific antibodies to respiratory viruses and the nucleic acid tests in children with community acquired pneumonia, and to guide clinical application of these tests.Methods:Data of 109 cases of children with community acquired pneumonia diagnosed as viral infection by real-time fluorescence quantitative PCR from nasopharyngeal swab specimens were collected. Their acute and convalescent serum specimens were collected and used to detect common respiratory viruses (respiratory syncytial virus, adenovirus, influenza A virus, influenza B virus and parainfluenza virus) specific IgM antibodies by indirect immunofluorescence method . The consistency between the results of virus serum specific antibody test and respiratory virus nucleic acid test was analyzed.Results:Among the 109 cases of community-acquired pneumonia patients with positive respiratory virus nucleic acid test, 14 cases (12.8%) were positive for virus specific IgM in the acute stage. Combining the results of serum virus specific antibody detection in the acute and convalescent phases, 17 cases (15.6%) were positive for virus specific IgM antibody. Consistency analysis showed that the acute phase serum specific IgM antibody test results of respiratory syncytial virus, adenovirus, influenza A virus, influenza B virus and parainfluenza virus were compared with the nucleic acid test results of respiratory specimens, and Kappa values were -0.033, -0.003, 0.053, -0.024, -0.053, respectively. Compared with the nucleic acid test results of respiratory tract specimens and the total serum specific IgM test results of respiratory syncytial virus, adenovirus, influenza A virus, influenza B virus and parainfluenza virus in the acute and convalescence stages, Kappa values were 0.040, -0.053, 0.065, -0.030 and -0.018, respectively. Kappa values were all less than 0.4, indicating that the consistency of detection results of the two methods is poor.Conclusions:The results of serum virus specific IgM antibody detection in children with community acquired pneumonia are poorly consistent with the results of viral nucleic acid detection in respiratory specimens, suggesting that serum virus specific antibody detection with the immunofluorescence method may not be suitable for the diagnosis of viral pathogens in children’s community acquired pneumonia.
