Non-replicating vaccinia virus Tiantan strain NTV induces early apoptosis
10.3760/cma.j.cn112866-20220208-00022
- VernacularTitle:非复制型痘苗病毒天坛株NTV诱发早期细胞凋亡
- Author:
Hang YUAN
1
;
Yabin WU
;
Jiao REN
;
Li ZHAO
;
Yamei DOU
;
Houwen TIAN
;
Wenling WANG
;
Wenjie TAN
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 国家卫生健康委员会医学病毒与病毒病重点实验室，北京 102206
- Keywords:
Vaccinia virus;
Replication defective;
Apoptosis;
Virus vector
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(2):136-140
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the cell morphological changes and related molecular mechanisms of non-replicating vaccinia virus NTV infection with human cells and to provide a scientific basis for the further optimization, transformation and application of NTV vectors.Methods:HeLa cells were infected with vaccinia virus Tiantan strain VTT and non-replicating vaccinia virus NTV, and the morphological changes of cells were observed. Then cells were harvested, and rRNA break levels were detected by electrophoresis and the molecular signals associated with apoptosis were detected by Western blotting, and the pathways and mechanisms of NTV-induced early apoptosis were preliminarily determined.Results:In this study, in terms of cell morphology, it was observed that cells infected with NTV were able to have cell rounding, wrinkles and other lesions at an earlier time compared with VTT. DAPI staining showed that NTV-infected nuclei exhibited high chromatin aggregation, marginalization, and disintegration over time. The rRNA fracture level test result indicate that rRNA has been broken and degraded after 16 hours of NTV infection. The Western blotting test result showed that the molecular signals of PARP, caspase-3 and caspase-9 that were stronger than in normal cells could be detected in NTV-infected HeLa cells, but there was no significant increase in caspase-8, while the result of VTT were the opposite of those in NTV.Conclusions:NTV can induce apoptosis in the early stage and provide a theoretical basis for the modification of vaccinia vectors.
