Whole-genome sequencing analysis of an imported novel coronavirus omicron BA.2 in Weihai city
10.3760/cma.j.cn112866-20220226-00044
- VernacularTitle:威海市一例输入性新型冠状病毒奥密克戎BA.2的全基因组测序分析
- Author:
Jinbo ZHANG
1
;
Xiang LI
;
Qi SUN
;
Zongyan SUI
;
Shuli SHAO
Author Information
1. 威海市疾病预防控制中心 检验科，威海 264200
- Keywords:
Novel coronavirus;
High-throughput sequencing;
Omicron BA.2
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(2):131-135
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify the genome-wide characteristics and variations of the novel coronavirus 2019(2019-nCoV) by combining high-throughput sequencing and bioinformatics techniques, to type and trace the virus.Methods:Pharyngeal swab sample of one 2019-nCoV infection case imported from Weihai city was selected for 2019-nCoV whole-genome sequence capture and NGS high-throughput sequencing. The obtained sequencing data were sequenced and analyzed using virus mutation analysis software, we classify viruses and build phylogenetic trees to track their potential sources.Results:The complete genome sequence of 2019-nCoV was obtained, with a total length of 29 808 bp and an average sequencing depth of 5 013.11×. A total of 51 nucleotide mutations were found in 8 coding regions (ORF1ab, S, ORF3a, E, M, ORF6, ORF7a and N). The result of virus typing showed that the virus belonged to Omicron BA.2. Evolutionary analysis showed that the virus sequence was in the same clade as the reference strains from Japan.Conclusions:The sequencing method and analysis result established in this study can be used for mutation analysis of 2019-nCoV and case tracing, which is of great significance for the prevention and control of COVID-19.
