Inhibitory effect of Niuhuang Qinggan Capsule on proliferation of 2019 novel coronavirus in vitro
10.3760/cma.j.cn112866-20220126-00017
- VernacularTitle:牛黄清感胶囊溶液体外抑制新型冠状病毒活性效果分析
- Author:
Xuexin LU
1
;
Shuangli ZHU
;
Yaning LIU
;
Xueying ZHAO
;
Tiejun DI
;
Youcai ZHOU
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 国家卫生健康委员会生物安全重点实验室，北京 102206
- Keywords:
Niuhuang Qinggan Capsule;
2019 novel coronavirus;
Coronavirus pneumonia;
Pneumonia treatment;
Drug screening
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(2):128-130
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Objective The inhibitory effect of Niuhuang Qinggan Capsule on 2019-nCoV virus was studied to provide experimental evidence for drug screening and treatment of 2019-nCoV acute respiratory disease.Methods:The 2019-nCoV virus GS048 strain was isolated and cultured by Vero-E6 cells. The solution of Niuhuang Qinggan capsule was incubated with virus in vitro to determine the change of virus titer. The effect of Niuhuang Qinggan capsule on the proliferation of 2019-nCoV virus was determined by cell culture medium method.Results:It was found that the concentration of 274.3 μg/ml was not toxic to the cells when the concentration of drugs was lower. When the drug was incubated with virus, the titer of virus decreased from 10 4.4TCID 50/ml to 10 2.8TCID 50/ml. In vitro 2019-nCoV virus culture experiment showed that the addition of the drugs reduced the cytopathy, and the logarithmic value of virus titer decreased by 40% -50%. Conclusion:In this study, in vitro inhibition experiments suggest that Niuhuang Qinggan capsule has the potential to inhibit the proliferation of 2019-nCoV. It can be used as a candidate drug to further verify the therapeutic effect of the 2019-nCoV acute respiratory disease.
