Evaluation of a parasite lactate dehydrogenase-based colloid gold-immunochromatography assay for diagnosis of Plasmodium falciparum
10.3321/j.issn:1673-4254.2005.07.001
- VernacularTitle:用乳酸脱氢酶为靶分子的免疫层析技术检测恶性疟原虫
- Author:
Ying-Song WU
1
;
La-Mei LEI
;
Ming LI
Author Information
1. 南方医科大学
- Keywords:
Plasmodium falciparum;
lactate dehydrogenase;
monoclonal antibodies;
colloid gold-immunochromatography assay
- From:
Journal of Southern Medical University
2005;25(7):761-765
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a colloid gold-immunochromatography assay (GICA) for detecting Plasmodiumfalciparum.Methods The monoclonal antibodies (mAbs) against lactate dehydrogenase ofP. falciparum (LDHpf) were screened for preparation of GICA strips. With microscopic examination of the blood smears and PCR test as control, GICAwas evaluated for its sensitivity, specificity and stability in the diagnosis of malaria in the outpatient clinics in China. Results Four hybridoma cell lines against LDHpf were prepared. Enzyme-linked immunosorbent assay demonstrated that the 4 mAbs reacted only with P. falciparum, but not with protein of normal human red cell, P. vivax, Toxoplasma gondii, or Schistosomajaponicam. All the 4mAbs recognized a 33 kD protein designated as LDHpf as shown by Western blot analysis. Compared with the microscopic examination of blood smears and PCR test, GICA had the sensitivities of 88.37% and 86.67% and the specificities of 95.65%and 97.78%, respectively. Concordance between microscopic examination and GICA for P. falciparum infection was91.55%.Conclusion GICA established in this study is simple, rapid, sensitive and specific for detecting P. falciparum, and is potentially useful in developing reagent kits for clinical use.
