The relationship between rs56061981 single nucleotide polymorphism of CXCL10 gene and the susceptibility and severity of HBV related chronic plus acute liver failure
10.3760/cma.j.cn112866-20200604-00181
- VernacularTitle:CXCL10基因rs56061981单核苷酸多态性与HBV相关慢加急性肝衰竭易感性及严重程度的关系
- Author:
Shaojun HUANG
1
;
Wenrong FU
;
Jingjing WANG
;
Zhengjiang CHENG
;
Xiuji CHEN
;
Xiaolin WANG
Author Information
1. 湖北文理学院附属襄阳市中心医院检验科，襄阳 441021
- Keywords:
HBV-related acute on chronic liver failure;
C-X-C motif chemokine ligand 10;
rs56061981;
Polymorphism
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(1):97-102
- CountryChina
- Language:Chinese
-
Abstract:
Methods:Whole blood and serum samples were collected from 86 patients with HBV-ACLF and 42 patients with chronic hepatitis B (CHB). Serum alanine aminotransferase (ALT), total bilirubin (TBil), creatinine (Cr), albumin (ALB) cholinesterase (CHE) and international standardized ratio (INR) were detected by routine method . The CXCL10 mRNA level of peripheral blood mononuclear cells was detected by fluorescence quantitative PCR. The genotype and allele of rs56061981 were detected by direct DNA sequencing. The genotype and allele frequencies between the ACLF and CHB groups were compared. The levels of ALT, TBil, INR, ALB, CHE, model for end-stage liver disease (MELD) and CXCL10 mRNA were compared between the two groups according to whether the T allele was carried.Results:There was no significant difference in sex, age, alcohol history, smoking history, HBV genotype, HBeAg status and HBV-DNA level between CHB group and HBV-ACLF group (P > 0.05). The frequency of CT+ TT genotype and the frequency of T allele at the rs56061981 locus of the CXCL10 gene in group ACLF were significantly higher than that in the CHB group ( χ2=4.83, P=0.03 and χ2=4.95, P=0.03). The difference is significant Genotype CT+ TT is a risk factor for CHB to develop into HBV-ACLF ( OR=2.897, 95% CI: 1.09~7.68). Allele T is a risk factor for CHB to develop into HBV-ACLF ( OR=2.746, 95% CI: 1.10~6.89). The plasma INR and MELD scores of ACLF individuals carrying T alleles were significantly higher than those carrying C alleles (t=2.63, P=0.013 and t=2.7, P=0.011). The difference is significant. Albumin (ALB) and cholinesterase (CHE) was significantly lower than those carrying C alleles (t=2.67, P=0.01 and t=3.545, P=0.001). The difference is significant. But there was no significant difference in serum TBL and ALT between the two groups. Conclusions:The polymorphism of CXCL10 gene rs56061981 is significantly correlated with the susceptibility and severity of ACLF. Allele T may be a susceptible gene of ACLF, and it can also be used as a predictor of the severity of the disease.
