Epidemiological situation and genotype analysis of astrovirus in infants in Qinghai province, from 2016 to 2018
10.3760/cma.j.cn112866-20200208-00019
- VernacularTitle:青海省2016—2018年婴幼儿星状病毒流行情况和基因型分析
- Author:
Guixiang LIU
1
;
Lixia FAN
;
Deng TIAN
;
Qiong XU
;
Shengcang ZHAO
;
Huayi ZHANG
;
Liyan FENG
;
Jingyu XIAO
Author Information
1. 青海省疾病预防控制中心，西宁 810000
- Keywords:
Infant;
Astrovirus;
Genotype
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(1):81-84
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the epidemic status about genotype of the astrovirus, and to provide the epidemic data and control epidemic of infectious disease.Methods:Screening for astrovirus positive nucleic acids was performed using an astrovirus real-time fluorescent PCR kit. RT-PCR amplification was performed with astrovirus-specific primers Mn289/270. The positive products were recovered and purified and directly sequenced. Sequence analysis and phylogenetic analysis of astrovirus sequences were performed using Clustal and MEGA3.0 biological software.Results:From 2016 to 2018, we collected 496 fecal specimens from children with diarrhea from different regions, of them 136 cases had viral diarrhea, and in 17 of them astrovirus (3.4%) was detected, there were more males than females. All the 17 cases were under 5 years of age, and 12 cases (70.58%) were younger than 1 year old. In regional distribution, samples from Xining accounted for 64.70% (11/17), Huangzhong county for 29.41% (5/17), Huangnan Prefecture for 5.88%(1/17). The positive cases were mainly found in April to June, and there was another peak from October to December, which was consistent with the time distribution of rotavirus in past years. Seven strains were HAstV-1, one strain was HAstV-2, and the homology among four strains of HAstV-1 was 99.0%-100%. The homology with the other two strains of the same type was 88.4-95.7%.Conclusions:The dominant genotype of Astrovirus was HAstV-1 in infants with diarrhea in Qinghai, meanwhile there was also HAstV-2.
