Viral infection and gene characteristics of human herpesvirus 6 in exanthema subitum cases in Gansu province, China in 2011
10.3760/cma.j.cn112866-20210924-00169
- VernacularTitle:2011年甘肃省幼儿急疹病例中人疱疹病毒6型感染情况及病毒基因特征分析
- Author:
Yangzi ZHOU
1
;
Jianfeng LIU
;
Xiaohong ZHAO
;
Yan ZHANG
;
Aili CUI
;
Ying CHEN
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 世界卫生组织西太平洋地区麻疹/风疹参比实验室 国家卫生健康委员会医学病毒和病毒病重点实验室，北京 102206
- Keywords:
Exanthema subitum;
Human herpesvirus 6B;
Gene characteristics
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(1):71-76
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clarify the infection status and gene characteristics of human herpesvirus 6 (HHV-6) in exanthema subitum cases in Gansu province in 2011.Methods:Throat swabs collected from 169 cases with exanthema subitum in Gansu province in 2011 were tested by the quantitative real-time PCR method. PCR amplification and sequence determination for U90 gene were performed for HHV-6 nucleic acid positive specimens and then the characteristics of U90 gene were analyzed in this study. Results:Sixty out of 169 samples from exanthema subitum cases were positive for HHV-6 nucleic acid, and 10 sequences of HHV-6B U90 gene were obtained. HHV-6 positive cases were mainly concentrated in infants under 2 years of age, with a median age of 9 months. Phylogenetic analysis showed that the sequences of HHV-6B could be divided into clades a and b. And then the clade a could be further divided into three lineages (lineage a-1, a-2 and a-3) with geographical clustering. The 10 HHV-6B sequences obtained in this study were scattered in different branches of the phylogenetic tree, belonging to lineages a-1 and a-2, and clade b. No gene recombination was found in these 10 sequences. Conclusions:Multiple HHV-6B clades/lineages were circulating in Gansu province, and the virus strains showed genetic polymorphism. Through this study, the gene characteristics of HHV-6B circulating in Gansu province were firstly clarified, which not only filled the gap of the HHV-6B molecular epidemiology in China, but also accumulated the data for global HHV-6B research.
