Molecular epidemiology of human astrovirus in infants and children with diarrhea in Jilin province from 2017 to 2020
10.3760/cma.j.cn112866-20211117-00201
- VernacularTitle:吉林省2017—2020年腹泻婴幼儿中人星状病毒分子流行病学特征
- Author:
Xiang LI
1
;
Shuang XU
;
Xinrong LU
;
Hongbo JIANG
;
Tingyu MENG
;
Yingwei MA
;
Donglin WU
;
Jingying ZHANG
;
Leilei WEI
Author Information
1. 吉林省疾病预防控制中心 吉林省公共卫生研究院，长春 130062
- Keywords:
Human Astrovirus;
Genotype;
Epidemiology;
Diarrhea
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(1):53-58
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the molecular epidemic characteristics and genes of human astrovirus (HAstV) in infantile diarrhea patients in Jilin province from 2017 to 2020.Methods:Fecal samples of hospitalized infants with diarrhea under 5 years of age from January 2017 to December 2020 were collected. The nucleic acids of fecal samples were amplified by reverse transcription-polymerase chain reaction (RT-PCR), the positive amplification products were sequenced, and the phylogenetic tree was constructed.Results:A total of 2 019 fecal samples were collected, of which 71 were positive for HAstV, with a positive rate of 3.5%. Among the cases with positive samples of HAstV, 42 had mixed infection with other viruses causing diarrhea, accounting for 59.2% of the total number of positive cases, half of which were mixed infection with rotavirus and HAstV. From 2017 to 2020, the positive rates of HAstV were 4.69%, 1.98%, 5.93% and 0.43% per year, respectively. There were two epidemic peaks every year and one epidemic peak year every two years. The positive rate of 36-47 months old was the highest, followed by 0-2 months old. A total of 55 sequences were obtained in this study. Phylogenetic tree analysis showed that they all belonged to classical HAstV, including 42 HAstV-1a subtypes, 8 HAstV-1b subtypes and 5 HAstV-5 subtypes.Conclusions:HAstV is one of the important pathogens of diarrhea in children under 5 years of age in Jilin province. The epidemic law has obvious seasonal and age differences. The dominant strain is HAstV-1a.
