Correlation between circulating T follicular helper cells and abnormal shift in B cell differentiation during HIV infection
10.3760/cma.j.cn112866-20210929-00176
- VernacularTitle:HIV感染者外周血Tfh细胞与B细胞异常分化相关性分析
- Author:
Xiaofan LU
1
;
Xin ZHANG
;
Huan XIA
;
Zhen LI
;
Yunxia JI
;
Hao WU
;
Tong ZHANG
;
Bin SU
Author Information
1. 首都医科大学附属北京佑安医院 感染性疾病临床研究中心 艾滋病研究北京市重点实验室，北京 100069
- Keywords:
T follicular helper cell;
B cell;
HIV infection
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(1):40-45
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the dynamics of circulating Tfh (cTfh) and B cells, and their relationship in individuals with acute (AHI) and chronic HIV infection (CHI).Methods:HIV infected subjects and HIV negative healthy controllers (HC) were enrolled from the men who have sex with men(MSM) high-risk cohort at Beijing Youan hospital. Multicolor flow cytometry was used for the frequency and absolute number analysis of cTfh cell and B-cell subsets with AHI, CHI, and HC.Results:Compared to HC, AHI resulted in an increase in cTfh cell levels and persisted into CHI, however, there was no significant difference between AHI and CHI (AHI vs HC: 2.09±1.48 vs 0.26±0.38, t=5.25, P<0.001; CHI vs HC: 2.26±1.35 vs 0.26±0.38, t=6.25, P<0.001; AHI vs CHI: 2.09±1.48 vs 2.26±1.35, t=0.40, P=0.449). Among B cell subsets, activated memory (AM), tissue-like memory (TLM), and plasmablast (PB) cell levels increased [AM(AHI vs HC: 3.59±1.77 vs 0.83±0.44, t=6.65, P<0.001; CHI vs HC: 3.99±2.49 vs 0.83±0.44, t=5.46, P<0.001); TLM(AHI vs HC: 11.05±4.96 vs 1.30±0.93, t=8.45, P<0.001; CHI vs HC: 13.91±6.59 vs 1.30±0.93, t=8.28, P<0.001); PB( AHI vs HC: 3.01±2.50 vs 0.43±0.26, t=4.47, P<0.001; CHI vs HC: 1.88±1.57 vs 0.43±0.26, t=3.97, P<0.001)], whereas resting memory (RM) and na?ve mature (NM) cell levels decreased in both AHI and CHI [RM(AHI vs HC: 20.06±9.74 vs 25.43±10.91, t=1.70, P=0.040; CHI vs HC: 15.70±8.47 vs 25.43±10.91, t=3.29, P=0.003); NM(AHI vs HC: 55.71±13.88 vs 66.26±11.71, t=2.90, P=0.004; CHI vs HC: 58.33±14.47 vs 66.26±11.71, t=1.94, P=0.006)]. The levels of cTfh cells were positively correlated with those of AM ( r=0.67, P<0.001), classical memory (CM) ( r=0.59, P=0.001), RM ( r=0.47, P=0.010), and PB ( r=0.65, P<0.001), and negatively correlated with those of NM B cells in AHI ( r=-0.55, P=0.003). However, cTfh cells did not show any relationship with B cell subsets except for the positive correlation with PB ( r=0.56, P=0.003) in CHI. Conclusions:HIV infection drives the expansion of cTfh cells, which may in turn leads to perturbations of B cell differentiation during AHI stage.
