Whole genome characterization of group A rotavirus among children under 5 years of age with acute diarrhea in Fuzhou sentinel hospital in 2020
10.3760/cma.j.cn112866-20211102-00191
- VernacularTitle:2020年福州哨点医院5岁以下住院腹泻儿童A组轮状病毒全基因组分析
- Author:
Hongmeng JIANG
1
;
Bingshan WU
;
Zhimiao HUANG
;
Yuwei WENG
;
Weidong LIN
;
Kuicheng ZHENG
Author Information
1. 福建医科大学公共卫生学院，福州 350122
- Keywords:
Group A rotavirus;
Whole genome;
Genetic ressortment
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(1):21-28
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the genome characteristics of group A rotavirus (RVA) strains among hospitalized children under 5 years of age with acute diarrhea in Fuzhou sentinel hospital in 2020.Methods:The ELISA method was used for screening RVA-positive stool samples of hospitalized children under 5 years of age, then 11 gene segments of RVA-positive samples were sequenced and typed after amplification by RT-PCR, and their homology and phylogenetic analysis were performed by Molecular Evolutionary Genetics Analysis (MEGA).Results:Twenty RVA whole genome sequences were successfully obtained, including 4 kinds of G/P gene combinations-G9P[8] (55%), G3P[8] (25%), G8P[8] (15%) and G2P[4] (5%). DS-1-like reassortant strains accounted for 40% of the whole genomes. Strains of the same type have high sequence homology, are closely related to the virus strains that currently circulating in the world. There are mutations at multiple important antigenic sites on VP7, VP4 and NSP4 fragments. The variation of amino acid substitutions of VP7, VP4 and NSP4 fragments is complicated, and there are many amino acid substitutions in the antigenic regions.Conclusions:G3P[8] and G8P[8] DS-1-like reassortants were detected for the first time in Fuzhou, amino acid substitutions were observed in the antigenic regions of the VP7, VP4 and NSP4 gene. Due to the emergence of uncommon DS-1-like reassortant strains and multiple important antigenic regions substitutions, it is necessary to continuously monitoring genome characteristics of RVA strains to provide scientific evidence for the pandemic prevention and vaccine immunization strategies.
