Effect of serum heat inactivation on the detection of 2019-nCoV antibodies
10.3760/cma.j.cn112866-20200312-00062
- VernacularTitle:血清热灭活对胶体金法检测新型冠状病毒抗体的影响
- Author:
Yuhai HU
1
;
Xiumei HU
;
Taixue AN
;
Peifu TIAN
;
Qiang LI
;
Xiaodan ZHENG
;
Jing WANG
;
Bo SITU
;
Dehua SUN
;
Yongyu RUI
;
Qian WANG
;
Lei ZHENG
Author Information
1. 武汉市汉口医院医学检验科 430010
- Keywords:
2019-nCoV;
Serum;
Heat inactivation;
Antibody;
Colloidal gold
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(6):719-722
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To assess the effect of serum heat inactivation on the detection of 2019 novel coronavirus (2019-nCoV) specific IgM and IgG antibodies by colloidal gold method.Methods:The serum specimens were collected from a total of 106 Coronavirus Disease 2019 (COVID-19) patients and 52 control subjects. Both the fresh serum and the heat inactivated serum samples from the same patient were detected simultaneously with the 2019-nCoV IgM and IgG antibodies detection kit (colloidal gold method). According to the patient′s onset time, the positive rates of antibodies production profile were calculated. The influence of heat inactivation on the detection rates of antibodies at different stages of disease after onset was analyzed.Results:The test results of the specimens of the healthy control group before and after inactivation were all negative. For the 106 specimens of COVID-19 patients, the detection rates of 2019-nCoV specific IgM and IgG antibodies were reduced after heating at 56 ℃ for 30 min. The positive rates of IgM antibodies significantly decreased from 66.04% (70/106) to 43.40% (46/106) ( χ2=22.042, P=0.000), while the positive rates of IgG antibodies slightly decreased from 81.13% (86/106) to 76.42% (81/106) ( χ2=0.800, P=0.063). Further analysis revealed that there was a significant difference in the positive rates of IgM antibodies before and after heat inactivation in the 3rd, 5th and 6th week after onset. However, there was no statistically significant difference in the detection rates of IgG antibodies before and after serum heat inactivation in different periods of onset. Conclusions:Heat inactivation significantly decreased the detection rates of 2019-nCoV specific IgM antibodies, which may lead to serological false negative results.
