2019-nCoV and herpes simplex virus type I were simultaneously isolated from a case of COVID-19
10.3760/cma.j.cn112866-20200731-00218
- VernacularTitle:从1例新型冠状病毒肺炎病例中同时分离到新型冠状病毒和单纯疱疹病毒I型
- Author:
Wei ZHANG
1
;
Xiaofang PENG
;
Huan ZHANG
;
Zhe LIU
;
Baisheng LI
;
Tianpeng HUANG
;
Changyun SUN
;
Lirong ZOU
;
Lijun LIANG
;
Qianfang GUO
;
Jie WU
;
Hui LI
;
Huanying ZHENG
Author Information
1. 广东省疾病预防控制中心，广州 511430
- Keywords:
2019 novel coronavirus (2019-nCoV);
Herpes simplex virus type 1 (HSV-1);
Virus isolation;
Co-infection
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(6):714-718
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the etiology of a confirmed case of Coronavirus Disease 2019 (COVID-19).Methods:The pharyngeal swabs, serum and nasal swabs of a case of COVID-19 were inoculated into Vero-E6 cell tubes for virus isolation. The cytopathic effect (CPE) were observed daily. Collecting cell’s isolation when CPE was over 75%, after repeated freezing and thawing for 3 times, the supernatant was centrifugally taken, and the images of the virus were obtained by transmission electron microscopic observation, and the nucleic acid of the virus was extracted, second generation sequencing and sequence evolution analysis were used to identify and type the virus strains.Results:One strain of 2019 novel coronavirus (2019-nCoV) was successfully isolated from the nasal swab of this case of COVID-19, and one strain of herpes simplex virus type 1 (HSV-1) was also successfully isolated from the throat swab of the same case.Conclusions:COVID-19 cases have the possibility of co-infection with 2019-nCoV and HSV-1.
