Application of BMD-PCR technology in rapid detection of 2019-nCoV nucleic acid
10.3760/cma.j.cn112866-20210224-00034
- VernacularTitle:BMD-PCR技术在新型冠状病毒核酸快速检测中的应用
- Author:
Yang JIAO
1
;
Hengwei WANG
;
Chong LI
;
Dan LIU
;
Yi ZHONG
;
Xiaoxuan HAN
;
Jianhong ZHAO
;
Zhi LYU
;
Shaocheng WANG
;
Lingli SUN
Author Information
1. 北京市朝阳区疾病预防控制中心 100021
- Keywords:
2019-nCoV;
Bubble mediated nucleic acid denaturation (BMD)-PCR;
Nucleic acid detection;
Limit of detection
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(6):664-668
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a sensitive and rapid nucleic acid detection method for 2019-nCoV that can be promoted in primary laboratories.Methods:The detection method for 2019-nCoV nucleic acid was established based on the Bubble Mediated Nucleic Acid Denaturation (BMD)-PCR technology with 2019-nCoV specific primers and probes. To validate this method, the laboratory-confirmed clinical samples (including 60 positives and 68 negatives of 2019-nCoV) were used and meanwhile the result were compared with the commercialized 2019-nCoV nucleic acid detection kits. The specificity of this method was validated by using 11 respiratory viruses and enteroviruses which cover common genotypes.Results:The BMD-PCR method showed good specificity in the detection of 2019-nCoV nucleic acid. It had no cross-reactivity with 11 common respiratory viruses and enteroviruses, and the limit of detection was less than 600 copies/ml. In addition, the qualitative result of 128 clinical samples were 100% consistent with the commercialized detection kits. Furthermore, the detection time can be shortened to 28 minutes.Conclusions:BMD-PCR for 2019-nCoV nucleic acid rapid detection could quickly diagnose and identify clinical samples, and could meet the current urgent needs of large-scale population and high-throughput screening which has important public health significance.
