Expression of α-enolase protein and its relationship with infection with HPV16/18 in laryngeal carcinoma patients
10.3760/cma.j.cn112866-20210628-00107
- VernacularTitle:喉鳞状细胞癌中α-烯醇化酶蛋白的表达及其与人乳头瘤病毒感染的关系
- Author:
Wei WEI
1
;
Yuezhang WU
;
Yuntao SONG
;
Guohui XU
;
Qi SHI
Author Information
1. 北京大学肿瘤医院暨北京市肿瘤防治研究所 头颈外科恶性肿瘤发病机制及转化研究教育部重点实验室 100042
- Keywords:
Laryngeal carcinoma;
α-enolase;
Human papillomavirus;
Immunohistochemistry
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(6):626-630
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the expression of α-enolase in laryngeal carcinoma patients and its relationship with the infection with HPV16/18.Methods:The expression of α-enolase protein in 98 cases was detected by immunohistochemical method and the relationship between α-enolase and clinical stage was analyzed. The expression of HPV16/18 was detected by PCR, sequencing and immunohistochemistry, the relationship between HPV16/18 expression and α-enolase expression was analyzed.Results:Immunohistochemical result showed that the total positive rate of α-enolase expression was 89.8%, and the total positive rate of HPV gene and protein detection was 40.8%. The localization of α-enolase protein tended to shift from cytoplasm and nucleus expression to more cytoplasmic expression only.Conclusions:With the progression of clinical course and HPV16/18 infection, the expression of α-enolase protein showed a tendency of localization to the cytoplasm. Detection of α-enolase protein can provide laboratory data reference for early diagnosis and treatment of laryngeal carcinoma.
