Evaluation of antibody detection methods based on the serum samples of clinically confirmed patients with the 2019 novel coronavirus infection
10.3760/cma.j.cn112866-20200324-00089
- VernacularTitle:基于微量病毒中和抗体试验的新冠肺炎临床确诊病例血清样本的抗体检测方法评价
- Author:
Juan SU
1
;
Dan LIANG
;
Wei LI
;
Yanling MO
;
Jianhong LIAO
;
Huanying ZHENG
;
Changwen KE
Author Information
1. 广东省疾病预防控制中心病原微生物检验所，广州 511430
- Keywords:
Colloidal gold immunoassay;
Fluorescence immunochromatography;
Micro neutralization test;
2019 Novel coronavirus
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(6):680-683
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect blood samples from clinically confirmed cases infected with the 2019 novel coronavirus (2019-nCoV) by fluorescence immunochromatography, colloidal gold immunoassay and micro neutralization test and compare differences in result and provide useful approaches to clinical and epidemiological investigation.Methods:The 2019-nCoV IgG/IgM antibody kit (Fluorescent immuno-chromatography) and the 2019-nCoV antibody test kit (Colloidal gold immunoassay) from Guangzhou Wanfu biotechnology Limited by Share Ltd, and the micro neutralization test established by a 2019-nCoV strain isolated by the laboratory in Guangdong Provincial Center for Diseases Control and Prevention were used to detect serum samples of clinically confirmed patients, in the Guangdong Province Second People′s Hospital, China.Results:A total of 113 serum samples from clinically confirmed cases infected with the 2019-nCoV were collected in Guangdong 2 nd People′s Hospital. The median age of the patients was 47.50 (32.00, 57.00) years and the gender ratio was 2.77∶1; The highest neutralizing antibody titer of micro neutralization test was 1∶1 024; Taking the result of micro neutralization test as gold standard, the sensitivity for colloidal gold immunoassay was greater than that of fluorescence immunochromatography (94.74% vs 82.46%), and the Kappa value for colloidal gold immunoassay and fluorescence immunochromatography was 85.84% and 75.24% respectively; at the same time, the negative predictive value and the positive predictive value for them were 94.44%, 91.53% and 83.87%, 92.16% respectively. Conclusions:In the serological method for the detection of the 2019-nCoV infection, the sensitivity and Kappa value for colloidal gold immunoassay were higher than those of fluorescent immunochromatography when the result of micro neutralization test was taken as the gold standard, which was more suitable for rapid detection of cases with the 2019-nCoV infection.
