Detection effect of novel coronavirus nucleic acid in throat swabs and nasal swabs
10.3760/cma.j.cn112866-20200309-00053
- VernacularTitle:咽拭子和鼻拭子标本新型冠状病毒核酸检测效果分析
- Author:
Lanlan ZHOU
1
;
Quande WEI
;
Liang JIAO
;
Huitao HUANG
Author Information
1. 珠海市疾病预防控制中心微生物检验所 519000
- Keywords:
COVID-19;
novel coronavirus 2019;
Nucleic acid detection;
Throat swab;
Nasal swab
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(6):710-713
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the detection effect of novel coronavirus 2019 nucleic acid in throat swabs and nasal swabs of COVID-19 cases.Methods:Throat swabs and nasal swabs were simultaneously collected from 541 cases of suspected cases and close contacts. Real time PCR was used to detect novel coronavirus 2019 nucleic acid. The positive rate of nucleic acid and Ct values of ORF1ab gene and N gene were analyzed. Results:A total of 43 cases were confirmed positive by nucleic acid detection result ; 35 cases were positive and the positive rate was 6.47% in throat swabs, while 33 cases were positive and the positive rate was 6.10% in nasal swabs. There was no significant difference in the positive rate between the two types of samples ( χ2=0.06, P=0.80). The diagnostic consistency of the two specimens was good ( Kappa=0.72). Ct values of ORF1ab and N genes between the two groups were not significantly different, either. Conclusions:There was no significant difference in the detection of novel coronavirus 2019 between throat swabs and nasal swabs from COVID-19 patients by real-time PCR.
