A simple and economic method for isolating hepatic lipocytes
10.3321/j.issn:1673-4254.2001.04.022
- VernacularTitle:一种简便又经济的肝脏贮脂细胞分离方法
- Author:
Yi-Jun ZHANG
1
;
Xi-Shan YANG
;
Ping-Sheng WU
;
Shu-Ren LIU
Author Information
1. Institute of Digestive Diseases of PLA
- From:
Journal of Southern Medical University
2001;21(4):304-306
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a simple and economic method for isolating rat hepatic lipocytes. Method Perfusion of the rat liver with pronase E and collagenase in succession was performed in cycles, and the hepatic lipocytes were isolated and purified by digesting the rat liver with DNase in the presence of the above 3 enzymes, followed by 11% Necodenz density gradient centrifugation. The resulted lipocytes were subjected to routine identification procedures and viability assessment. Results The yield of the lipocytes from each rat was 2×107 to 3×107, with viability over 95% and purity above 90%. Conclusion The method adopted in this study is simple, economic and highly productive.
