Detection and genetic analysis of newer enteroviruses EV-A89 and EV-C96 in environmental sewage, in Jinan
10.3760/cma.j.cn112866-20210406-00057
- VernacularTitle:济南环境污水中肠道病毒A89型和C96型的基因特征分析
- Author:
Rongrong HUANG
1
;
Xiaojuan LIN
;
Feng JI
;
Zexin TAO
;
Aiqiang XU
Author Information
1. 山东大学齐鲁医学院 公共卫生学院，济南 250012
- Keywords:
New enterovirus;
Environmental surveillance;
Phylogenetic analysis;
Next generation sequencing
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(5):530-535
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore newer enteroviruses (EVs) circulating in China and research their genetic characterization.Methods:Sewage samples were collected in Jinan in January and July of 2018, respectively. PCR amplification of the P1 coding region of EV and next-generation sequencing (NGS) were conducted. Homologous and phylogenetic analyses were performed on the newly identified newer EVs.Results:The EV-A89 (n=2) and EV-C96 (n=1) nucleic acid sequences were detected in the sewage in January and July, respectively and obtained complete P1 coding sequence. The two EV-A89 sequences had 6.1% nucleotide divergence among themselves, and had 88.2%-95.3% homologies with other strains. No close genetic relationship was obtained with the sequences from Xinjiang, China and those from other countries. The EV-C96 sequence in this study had 76.2%-89.4% nucleotide similarities with other isolates throughout the world. Phylogenetic analysis showed that global EV-C96 strains were divided into 5 branches, i. e, A to E. Except for the Shandong 1991 isolate, the other Shandong and domestic strains were all located in branch D.Conclusions:This study added the molecular epidemiologic data of newer enteroviruses EV-A89 and EV-C96, and provides basic data for future research on the epidemic trend of EV-A89 and EV-C96 as well as their association with diseases.
