Serological and genotypic surveillance of mumps in Fujian province, 2018
10.3760/cma.j.cn112866-20201209-00300
- VernacularTitle:2018年福建省流行性腮腺炎血清学及病毒基因型监测
- Author:
Hairong ZHANG
1
;
Xiuhui YANG
;
Ruihong WU
;
Suhan ZHANG
;
Weiyi PAN
;
Yong ZHOU
;
Dong LI
Author Information
1. 福建省疾病预防控制中心 福建省人兽共患病重点实验室，福州 350001
- Keywords:
Mumps virus;
Antibody levels;
Genotype
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(5):524-529
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the level of mumps virus (MuV) IgG antibody of healthy population and the genotyping of MuV in Fujian province in 2018.Methods:MuV IgG antibody of healthy population was detected by enzyme-linked immunosorbent assay (ELISA). Small hydrophobin (SH) gene of MuV was genotyped in pharyngeal swab and cell cultures of mumps patients using reverse transcription polymerase chain reaction (RT-PCR). The phylogenetic tree by SH sequences was constructed to identify MuV genotype.Results:A total of 4 925 people completed antibody testing, the positive rate of MuV IgG antibody was 78.58%(3780/4925), and the geometric mean concentration (GMC) was 245.83 IU/ml. There was no statistically significant difference in the positive rate of antibodies between people of different genders, while GMC had statistically significant differences ( χ2=4.295, P=0.117; Z=-2.220, P=0.026). There were significant differences in the positive rate of antibodies and GMC between people in different regions and age groups. Especially in infants and 12-15 years old group of people, the MuV IgG antibody positive rate and GMC were at low levels. The antibody positive rate and GMC of people with a history of mumps-containing vaccine (MuCV) immunization were higher than those without a history of MuCV immunization and those with unknown MuCV immunization history ( χ2=259.315, P<0.001; Z=-16.319, P<0.001). Eight strains of MuV were isolated from the mumps outbreak, which were all F genotypes. Conclusion:The immune level of mumps in infants and young children in Fujian province was low, and the 12-15 age group is the focus of attention. The epidemic strains of MuV in Fujian province in 2018 were mainly F genotype strains.
