Molecular genetic analysis of standard strains for functional antibody detection of pneumococcal polysaccharide vaccine
10.3760/cma.j.cn112866-20210624-00105
- VernacularTitle:检测肺炎球菌疫苗功能性抗体的标准株分子特征分析
- Author:
Yang HUANG
1
;
Xiao XU
;
Kang LI
;
Jiangjiao LI
;
Jichun SHI
;
Rui ZHENG
;
Wenyuan SUN
;
Qiang YE
Author Information
1. 中国食品药品检定研究院中国医学细菌保藏管理中心，北京 102629
- Keywords:
Pneumococcal polysaccharide vaccine;
Functional antibody detection;
Standard strains;
Molecular typing
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(5):509-513
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze molecular genetic characteristics of standard strains for functional antibody detection of pneumococcal polysaccharide vaccine, and provide the foundation for clinical research of pneumococcal polysaccharide vaccine. Methods:The 24 strains for functional antibody detection of pneumococcal polysaccharide vaccine were detected by PCR amplification and sequencing using standard strain 16S rRNA gene fragment, and the molecular typing of the related strains was performed by multi locus sequence analysis (MLST) and pulsed field gel electrophoresis (PFGE). Results:The result of 16S rRNA gene sequence alignment showed that the similarity between the gene sequences of the 24 strains and that of NCTC 7465 (LN831051) ranged from 98.35% to 99.86%, and the base difference was 2-23 bp. Multi locus sequence analysis showed that the ST types of 24 standard strains were different. PFGE analysis showed that 24 standard strains had good isolation effect from DNA fragments of 100 kb-1 000 kb, and each serotype had its own characteristic bands (12-15 fragments).Conclusions:This study analyzed the molecular genetic characteristics of the standard strains for the detection of pneumococcal functional antibodies for the first time, and may provide a reference for the quality control method of the standard strains for the subsequent detection of pneumococcal functional antibodies.
