Effect of eight kinds of inactivated virus preservation solutions on the stability of virus nucleic acid
10.3760/cma.j.cn112866-20210714-00121
- VernacularTitle:八种灭活型病毒保存液对病毒核酸稳定性的影响
- Author:
Yi YANG
1
;
Qinghe SONG
;
Xia WANG
;
Lianhua DONG
Author Information
1. 中国计量科学研究院前沿中心，北京 100029
- Keywords:
Virus preservation solution;
Avian influenza virus;
2019-nCoV;
Pseudovirus reference material;
Reverse transcription real-time quantitative PCR;
Reverse tr
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(5):500-504
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the protective effects of 8 different inactivated virus preservation solutions on viral nucleic acids.Methods:Eight commercial inactivated virus preservation solutions were evaluated by adding the pseudovirus standard material for SARS-CoV-2 and avian influenza virus, respectively. Two control groups (physiological saline and phosphate buffer) were set up at the same time. Reverse transcription real time quantitative PCR (RT-qPCR) and reverse transcription digital PCR (RT-dPCR) were used to quantify the nucleic acids extracted from the above treatment at 0 h, 6 h, 12 h, 24 h, 48 h, 72 h at 24 ℃ and 37 ℃.Results:Four commercial virus preservation solutions (A, E, G, and H) could effectively protect the viral nucleic acids under different temperature and time conditions. However, the preservation solution B and C could not provide good protection after 12 h at 37 ℃. The protection effect of preservation solution D significantly decreased after 6 h at both 24 ℃ and 37 ℃. Preservation solution F could not provide any protection on viral nucleic acid.Conclusions:The poor protective effect of virus preservation solution can lead to false negatives in nucleic acid testing. It is recommended to control the quality of the virus preservation solution during nucleic acid testing.
