Analysis of epidemiological characteristics of respiratory pathogens in children by multiple RT-PCR based on capillary electrophoresis
10.3760/cma.j.cn112866-20191212-00200
- VernacularTitle:基于多重RT-PCR检测法的儿童呼吸道病原体流行特征
- Author:
Wenyuan LIU
1
;
Yizhi ZHANG
;
Chengbo ZHOU
;
Junhua WU
;
Wenbo LU
Author Information
1. 宁波市妇女儿童医院检验科 315012
- Keywords:
Children;
Respiratory pathogens;
Multiple RT-PCR
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(4):467-471
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the epidemiological characteristics of children respiratory pathogen infection in Ningbo city, and provide scientific reference for clinical prevention, control, diagnosis and treatment.Methods:A total of 3 103 specimens were collected from the children with acute respiratory infections in Ningbo Women and Children′s Hospital from July to October 2019. Thirteen common respiratory pathogens were detected by multiple reverse transcription-polymerase chain reaction (RT-PCR) based on capillary electrophoresis, and the result were analyzed.Results:Of the 3 103 specimens, 2 053 were positive for respiratory pathogens, with a total positive rate of 66.16%. The detection rates of Mycoplasma pneumoniae (Mp) 33.19% (1 030/3 103), human rhinovirus (HRV) 24.20% (751/3 103), and human adenovirus (HADV) 8.77% (272/3 103) were in the first three. The positive rates of pathogens in the male and female children were 65.70% (1 157/1 761) and 66.77% (896/1 342), respectively, and the difference was not statistically significant( χ2=0.386, P=0.535); the positive rates in the age groups of ≤1 year old, 1~≤3 years old, 3~≤6 years old and 6~≤16 years old were 58.02% (626/1 079), 74.37% (589/792), 73.78% (543/736) and 59.48% (295/496), respectively, and the difference among the groups was statistically significant( χ2=84.770, P=0.000); the positive rates in July to October were 71.64% (341/476), 68.81% (536/779), 67.01% (648/967) and 59.93% (528/881), respectively, the difference was statistically significant( χ2=24.395, P=0.000); the single infection rate was 52.30% (1 623/3 103), and the mixed infection rate of two or more pathogens was 13.86% (430/3 103), most of which were mixed infections of two pathogens. Conclusions:The main respiratory pathogens of children in Ningbo city were Mp and HRV, and the detection rates of respiratory pathogens varied among different ages and months.
