Preparation and detection of antibody to encephalomyocarditis virus 3C protease
10.3760/cma.j.cn112866-20201117-00289
- VernacularTitle:脑炎心肌炎病毒3C蛋白酶抗体的制备和检测
- Author:
Qinqin SONG
1
;
Gongqi LI
;
Jun HAN
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 传染病预防控制国家重点实验室 传染病诊断与治疗创新协作中心，北京 102206
- Keywords:
Encephalomyocarditis virus;
3C protease;
Monoclonal antibodies
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(4):463-466
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare encephalomyocarditis virus (EMCV) 3C protease antibody and detect response sites of these antibody.Methods:Prokaryotic expression vector pGEX-2T-3C was constructed and expressed. Then BALB/c mice was immunized with 3C protein and antibodies were obtained. The specificity of the antibody binding to 3C protein was detected by immunofluorescence and Western blotting. Three different fragments of EMCV 3C were expressed and detected with monoclonal antibodies through Western blotting.Results:The antibody reacted specifically with 3C proteins of prokaryotic expression, and bound well with 3C protein of BHK cells infected with EMCV virus through Western blotting and immunofluorescence. The reaction between fragments of 3C protein and the antibody revealed that the antibody could react with the C-terminal of 3C protein.Conclusions:The prepared monoclonal antibody recognized C terminal of EMCV 3C protein.
