Roles of N-methyl-D-aspartate and non-N-methyl-D-aspartate receptors in the respiratory rhythmical discharge of the hypoglossal nerve in the brainstem slice from neonatal rats
10.3321/j.issn:1673-4254.2001.04.004
- VernacularTitle:N-甲基-D-天门冬氨酸和非N-甲基-D-天门冬氨酸类受体在新生大鼠延髓脑片呼吸节律性放电中的作用
- Author:
Bing-Xing PAN
1
;
Zhong-Hai WU
;
Ning-Qian WANG
Author Information
1. First Military Medical University
- From:
Journal of Southern Medical University
2001;21(4):251-254
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of N-methyl-D-aspartate (NMDA) and non-NMDA receptors in the generation and modulation of basic respiratory rhythm. Methods Respiratory rhythmical discharge activity (RRDA) of the hypoglossal nerve was recorded by suction electrode on the brainstem slices isolated from the neonatal rats, and the effects of the excitatory amino acids and its antagonists on the RRDA were investigated by adding these drugs into the modified Kreb's solution perfusing the brainstem slices. Results After application of the non-NMDA receptors agonist KA, it was found that the respiratory cycle and the expiratory time were slightly lengthened, but the NMDA receptor agonist NMDA had no effect on the RRDA. Both of the mutual antagonist DNQX and AP5 remarkably decreased the discharge frequency and the integral amplitude, accompanied by the shortening of the inspiratory time; DNQA simultaneously shortened the respiratory cycle and the expiratory time. Conclusion During the generation and the modulation of the mammalian respiratory rhythm, NMDA receptors act mainly to regulate the amplitude of the respiratory activity, and the non-NMDA receptors can not only affect the respiratory amplitude but also modulate the respiratory rhythm.
