Studies on purification and renaturation of recombinant human IL-2/GM-CSF fusion protein
10.3321/j.issn:1673-4254.2001.04.002
- VernacularTitle:重组人白细胞介素-2/ 粒细胞-巨噬细胞集落刺激因子融合蛋白的纯化及复性研究
- Author:
Xing-Mei LINLAI
1
;
Ming-Qian ZHOU
;
Ze-Hong CHEN
;
Zhi-Ming HU
;
Jing LIU
;
Shu-Qi HUANG
;
Xiao-Ning WANG
Author Information
1. 第一军医大学
- From:
Journal of Southern Medical University
2001;21(4):245-247
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the purification and renaturation of recombinant human IL-2/GM-CSF fusion protein. Methods After the inclusion bodies were isolated, the optimal conditions of denaturation and renaturation were studied. The renatured recombinant human IL-2/GM-CSF was purified by DEAE-Sepharose FF ion-exchange chromatography. Results The purified product manifested biological activity with purity of up to 95%. Conclusions The procedures employed in this study is simple and reliable.
