Isolation, identification and genomic sequence analysis of the first yellow fever virus strain in Fujian province
10.3760/cma.j.cn112866-20210521-00083
- VernacularTitle:福建省首株黄热病毒株的分离鉴定和全基因组序列分析
- Author:
Jinzhang WANG
1
;
Qi LIN
;
Tingting YU
;
Naipeng KAN
;
Libin YOU
;
Yuwei WENG
Author Information
1. 福建省疾病预防控制中心 福建省人兽共患病研究重点实验室，福州 350001
- Keywords:
Yellow fever;
Yellow fever virus;
Virus isolation;
High-throughput sequencing;
Phylogenetic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(4):429-432
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To isolate and identify the yellow fever virus (YFV) from the specimens of the imported yellow fever (YF) cases in Fujian province in 2016.Methods:Sixteen positive YFV nucleic acid samples including serum, urine and saliva were inoculated into C6/36 cells, respectively. The isolated strain was identified by YFV real-time RT-PCR. The complete gene sequence of this strain was obtained by high-throughput next-generation sequencing, and the phylogenetic tree was drawn.Results:Only one strain was isolated from the serum of one case three days after onset, and identified as a YFV strain by real-time RT-PCR. BLAST analysis showed that the complete gene sequence of this strain was identical to the strain CNYF01R/2016(KX268355) isolated from the first YF imported case in China in 2016. The phylogenetic tree showed that this strain belonged to the same phylogenetic branch as the epidemic strain Angola71 in Angola in 1971, and was significantly different from the 17D vaccine strain (X03700), indicating that the YFV strain isolated in this study belonged to the wild YFV strain of Angola genotype.Conclusions:An Angola genotype YFV strain was successfully isolated from samples of imported YF cases in Fujian Province in 2016.
