Genetic characteristics of VP1 region of two Coxsackievirus A12 strains in Yunnan province
10.3760/cma.j.cn112866-20200923-00253
- VernacularTitle:云南省2株柯萨奇病毒A12型VP1区基因特征分析
- Author:
Xiaofang ZHOU
1
;
Jianping CUN
;
Lili JIANG
;
Bingjun TIAN
;
Nan LI
;
Xiaoqing FU
;
Yihui CAO
Author Information
1. 云南省疾病预防控制中心，昆明 650022
- Keywords:
Hand, foot and mouth Disease;
Coxsackievirus A12;
VP1 gene
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(4):408-411
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To do genetic analysis on the VP1 gene of 2 Coxsackievirus A12 (CV-A12) isolated from hand, foot and mouth disease (HFMD) surveillance in Yunnan province.Methods:Coxsackievirus isolation was carried out in RD cells from the clinical samples. CV-A12 strains were identified by realtime RT-PCR and sequencing technology from the positive cultures. The VP1 region of the CV-A12 strain was further sequenced. The VP1 gene sequences were initially aligned and then used to construct the phylogenetic tree with GenBank reference strains.Results:The two CV-A12 strains had the highest homology with the Yunnan reference strains in 2018 and the amino acids in VP1 region had specific mutations with other cluster reference strains at multiple sites.Conclusions:The CV-A12 in the HFMD cases in Yunnan province has occured regional specific mutation in VP1 gene.
