Establishment of double antibody sandwich ELISA for detection of 2019-nCoV antigen
10.3760/cma.j.cn112866-20210311-00045
- VernacularTitle:新型冠状病毒抗原检测双抗体夹心ELISA方法的建立
- Author:
Yuanyuan QU
1
;
Wei WU
;
Qiangling YIN
;
Chuan LI
;
Jiandong LI
;
Mifang LIANG
;
Lina SUN
;
Shiwen WANG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所，北京 102206
- Keywords:
Severe acute respiratory syndrome coronavirus 2 (2019-nCoV);
Monoclonal antibody;
Double antibody sandwich ELISA;
Antigen detection
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(3):357-360
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore an early, rapid and accurate detection method of severe acute respiratory syndrome coronavirus 2 (2019-nCoV) antigen and improve the detection rate of patients with 2019-nCoV.Methods:We detected the characteristics of monoclonal antibodies (mAbs) against 2019-nCoV generated previously and established a double antibody sandwich ELISA against S protein of the virus.Results:All 12 mAbs against 2019-nCoV screened in our previous study were able to recognize purified virion and S protein. They were good candidates for detecting antibody to 2019-nCoV antigen. A double antibody sandwich ELISA established in this study could rapidly and effectively detect 2019-nCoV S protein with high sensitivity. The detection limit of 2019-nCoV S protein was lower than 1 ng/ml.Conclusions:This method could specifically detect 2019-nCoV antigen, and provided a meaningful reference for early, sensitive and specific diagnosis of COVID-19 infection.
