Correlation between DEPDC5 rs5998152 single nucleotide polymorphism and risk of HCV-related liver diseases
10.3760/cma.j.cn112866-20200318-00075
- VernacularTitle:DEPDC5 rs5998152单核苷酸多态性与丙型肝炎病毒相关疾病发生风险的相关性分析
- Author:
Kunyan QIAO
1
;
Shitian ZHANG
;
Rui SU
;
Wei HOU
;
Fengmei WANG
Author Information
1. 天津市第二人民医院 天津市肝病医学研究所 300192
- Keywords:
DEPDC5;
Single nucleotide polymorphism;
Hepatitis C;
Liver cirrhosis;
Hepatocellular carcinoma
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(3):300-304
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To examine the correlation between DEPDC5 rs5998152 variants and the risk of hepatitis C virus (HCV) related liver diseases onset. Methods:Patients with chronic hepatic diseases diagnosed as HCV infection in Tianjin Second People′s Hospital from September 2016 to July 2017 were enrolled in the study and were divided into chronic hepatitis C (CHC) group, CHC related liver cirrhosis (LC) group and hepatocellular carcinoma (HCC) group. DEPDC5 rs5998152 was genotyped using the matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) assay and the case data were reviewed. T test, analysis of variance (ANOVA), and non-parametric test were used to perform the comparison of the quantitative data between groups according to normally distributed or not. Chi-square test was used to examine the different distribution of enumeration data between groups. Logistic regression analysis was employed to analyze the correlation between the genetic polymorphism and risk of LC and HCC. Results:A total of 147 patients were included in this study, with 55 in CHC group, 54 in LC group and 48 in HCC group. The levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST), α-fetoprotein (AFP), total bilirubin and rate of hepatic encephalopathy were significantly higher in LC and HCC groups compared to CHC group( P<0.05). And the levels of AFP and total bilirubin were significantly higher in HCC group than LC group ( P<0.05). There was no significant difference among the three groups in terms of DEPDC5 rs5998152 genotype distribution ( P > 0.05). The frequency of the C allele at DEPDC5 rs5998152 was higher in LC and HCC subjects than in CHC patients ( P<0.05) and the Logistic analysis indicated that CHC individuals with C allele and TC+ CC genotypes showed higher risk of LC and HCC compared with those with T allele and TT genotype ( P<0.05). In addition, the difference of DEPDC5 rs5998152 allele frequency was not significant between LC and HCC groups and it was not correlated with risk of HCC for LC patients. Conclusions:DEPDC5 rs5998152 may be a risk factor of progression to LC and HCC in the Chinese Han patients with CHC.
