Preparation of virus-like particles modified with RGD peptide and targeted delivery of doxorubicin
10.3760/cma.j.cn112866-20200408-00116
- VernacularTitle:RGD肽修饰病毒样颗粒靶向递送阿霉素的初步研究
- Author:
Rui FANG
1
;
Yunlong WANG
;
Yinyin YU
;
Yulin LI
;
Jichuang WANG
;
Yiqing ZHANG
;
Lei CHENG
;
Shegan GAO
;
Jiangang WANG
;
Sanqiang LI
Author Information
1. 河南科技大学，洛阳 471023
- Keywords:
Virus-like particle;
Doxorubicin;
Breast cancer
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(3):296-299
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the biological effects of the targeted nanocomposite on breast cancer 4T1 cells with hepatitis B virus-like particles (HBc-RGD-VLPs) as a carrier for the delivery of anti-cancer drugs, and to provide a new theoretical basis for reducing the toxicity of doxorubicin (DOX) anti-tumor drugs and changing the path of administration.Methods:The hepatitis B pseudoviral particles prepared in the early stage of this laboratory enveloped DOX to form a target nanocomposite HBc-RGD-VLPs/DOX. The homogeneity and morphology of particles were detected by transmission electron microscopy and granular size analyzer, and applied to 4T1 cells for in vitro bioactivity exploration.Results:The structure of the target nanocomposite HBc-RGD-VLPs/DOX was detected by means of a transmission electron microscope, in a homogenous form, and the particle size distribution was 30-35 nm. In vitro cell experiments showed that the safety of target vector HBc-RGD-VLPs was better, the cell survival rate was more than 80%, and the HBc-RGD-VLPs/DOX after encapsulation had a significant inhibitory effect on the growth of 4T1 cells, and the effective inhibitory concentration (IC50) for half of 4T1 tumor cells was 1.445 g/ml. Fluorescence microscopy showed that HBc-RGD-VLP/DOX can be specifically targeted to tumor cells relative to the isolated DOX.Conclusions:The safety of target vector HBc-RGD-VLPs is better, HBc-RGD-VLPs/DOX showed good proliferation inhibitory effect and certain tumor-targeting effect on tumor cells.
