Hemagglutinin gene characteristics of the H9N2 avian influenza virus from the first patient infected with this virus in Jiangsu province
10.3760/cma.j.cn112866-20201019-00268
- VernacularTitle:江苏省首例人感染H9N2禽流感的发现及病毒血凝素基因特征分析
- Author:
Zefeng DONG
1
;
Cheng LIU
;
Xuerong YA
;
Di WANG
;
Qiang SHEN
;
Yu XIA
Author Information
1. 苏州市疾病预防控制中心 215004
- Keywords:
Avian Influenza;
H9N2 virus;
Hemagglutinin;
Mutation;
N-Glycosylation
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(3):276-279
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To disclose the epidemiological characteristics of the first human case of H9N2 subtype avian influenza and genetic characteristics of hemagglutinin (HA) gene of H9N2 avian influenza virus in Jiangsu province.Methods:Reverse transcription polymerase chain reaction (RT-PCR) assay was used to detect the nucleic acid of influenza virus A/B and various subtypes from throat swab specimens of patients with acute and severe respiratory infections of unknown cause. Sanger method was used to sequence the vial HA gene of H9N2 positive specimens. N-J phylogenetic tree of HA gene was constructed by using MEGA X software.Results:The first laboratory confirmed case of human infection with H9N2 avian influenza virus in Jiangsu province in March 2019 was reported by Suzhou Center for Disease Control and Prevention. Sequence analysis of the HA gene showed that the A/Suzhou/S683/2019 (H9N2) strain belonged to Y280-like sub-lineage of the Eurasian lineage, which had 99.23% nucleic acid identity with A/chicken/Shanghai/S1171/2018 (H9N2) strain. The amino acid sequence of the HA protein cleavage site is PSRSSR↓GLF, and the key amino acid in receptor binding site shared Q226L mutation. The location of potential glycosylation sites of HA protein of A/Suzhou/S683/2019 (H9N2) have changed.Conclusions:The H9N2 avian influenza virus obtained from the first case in Jiangsu province has low pathogenicity, but it may have acquired part of the ability to adapt to human hosts.
